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KMID : 1161420170200030211
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Journal of Medicinal Food
2017 Volume.20 No. 3 p.211 ~ p.222
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Time Course Effect of R-Alpha-Lipoic Acid on Cellular Metabolomics in Cultured Hepatoma Cells
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Ikuta Naoko
Chikamoto Keita
Asano Yuya
Yasui Yoshiaki
Yokokawa Haruka
Terao Keiji
Rimbach Gerald
Matsugo Seiichi
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Abstract
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Alpha-lipoic acid (LA) is a powerful antioxidant. LA has two enantiomers, R(+)-LA (R-LA) and S(?)-LA (S-LA). Of these, R-LA is naturally occurring and an essential cofactor in energy metabolism. R-LA treatment has been reported to affect glucose metabolism in rat hepatoma cells. This study analyzed the time course of metabolite levels in LA-treated cultured H4IIEC3 rat hepatoma cells, including a specific evaluation of the effect of R-LA and the enantioselectivity of LA. Principal component analysis showed that this experiment was well designed to observe enantioselectivity. R-LA treatment was found to inhibit the glycolysis and Thr-Gly-Ser pathways, as well as lactic acid production, leading to the inhibition of gluconeogenesis in starved H4IIEC3 cells. This study may provide mechanistic insight into how R-LA induces apoptosis in hepatoma cells.
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KEYWORD
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amino acids, enantioselectivity, glyconeogenesis, hepatoma cells, H4IIEC3, lipoic acid, metabolomics
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